Anti-CD56/NCAM1 Monoclonal Antibody for FC - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1, CD56) is a cell adhesion protein involved in neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurites.
• Acts as a receptor for rabies virus and Zika virus during microbial infection.
• Subcellular localization includes cell membrane (found on isoforms with transmembrane or GPI-anchor) and secreted isoforms.
• Belongs to the immunoglobulin superfamily, containing Ig-like domains, disulfide bonds, and is heavily glycosylated.
• Post-translational modifications include phosphorylation and glycosylation, contributing to its adhesive functions.
• Alternative splicing generates multiple isoforms with distinct membrane anchoring modes and tissue distributions.
• The canonical isoform has an unmodified molecular weight of ~94.6 kDa, with observed migration around 95 kDa due to glycosylation.

Experimental Guidance and Technical Tips

• This recombinant rabbit monoclonal antibody is designed for flow cytometry (FC) and targets the extracellular region (aa 20-413) of human NCAM1.
• For robust FC detection, use cell types known to express CD56, such as natural killer (NK) cells, neural cells, or subsets of leukocytes.
• Optimize antibody dilution by performing a titration series in the relevant experimental system to determine the optimal staining index.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and exclusion of non-specific binding.
• Validate staining specificity by using CD56-positive and negative cell lines or by pre-blocking with recombinant NCAM1 protein.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers and researchers with comprehensive access to high-quality IVD raw materials, streamlining development from discovery to clinical application.
• For neurobiology, immunophenotyping, or viral entry studies targeting NCAM1, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents.
• Our one-stop sourcing model includes rare target raw-material procurement and technical consulting to accelerate your assay development.