Anti-CD55 Rabbit Polyclonal Antibody for WB, ELISA - P08174

Core Product Specifications and Parameters

Biological Background: CD55 Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored glycoprotein that regulates the complement system by inhibiting the formation and stability of C3 and C5 convertases.

Related references: PMID:7525274 PMID:28657829

• It binds to C4b and C3b fragments, preventing the assembly of C4b2a and C3bBb amplification convertases, thereby protecting host cells from complement-mediated damage.

Related references: PMID:7525274 PMID:28657829

• CD55 contains four short consensus repeats (SCRs), also known as sushi domains, which mediate its interaction with complement components.

• The protein is expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins, and soluble forms are found in body fluids.

• CD55 acts as a receptor for several picornaviruses, including Coxsackievirus A21 and B, Human enterovirus 70 and D68, and echoviruses, facilitating viral entry.

• Mutations in CD55 cause CHAPLE syndrome, a rare autosomal recessive disorder characterized by early-onset protein-losing enteropathy and complement hyperactivation.

• Post-translational modifications include N-glycosylation, disulfide bond formation, and addition of a GPI anchor that tethers it to the cell membrane.

• Alternative splicing generates multiple isoforms, including membrane-bound and secreted variants.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 40-300) encompasses the extracellular sushi repeat domain; antibodies raised against this region are expected to recognize native CD55 and may be suitable for applications requiring detection of the mature protein.

• For Western blotting, the predicted molecular weight is 41 kDa, but glycosylation can cause a shift to 50–70 kDa; validate with a relevant positive control, such as human peripheral blood mononuclear cell lysate.

• The antibody is reported to cross-react with mouse; however, independent validation in the experimental system is recommended.

• For ELISA, consider using the antibody as a capture or detection reagent in sandwich assays, paired with another CD55-specific antibody to avoid competition for epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers and researchers, offering a comprehensive range of raw materials and technical support from concept to clinic. This anti-CD55 polyclonal antibody supports complement biology studies and the development of diagnostic assays for complement-mediated disorders. Beyond this product, CamelBio can source validated antibody pairs, bulk ancillary reagents, and customized raw materials to accelerate your diagnostic pipeline.