Anti-CD53 Rabbit Polyclonal Antibody for WB, ELISA - P19397

Core Product Specifications and Parameters

Biological Background: CD53 Function and Localization

• CD53, also known as tetraspanin-25 (Tspan-25) or MOX44, is a member of the tetraspanin superfamily and a structural component of tetraspanin-enriched microdomains (TERMs), which facilitate receptor clustering and signal transduction (PubMed:28487417). Related references: PMID:28487417
• It plays a critical role in monocyte and B-cell activation, contributing to immune cell signaling pathways (PubMed:8335905). Related references: PMID:8335905
• CD53 is an essential regulator of B-cell development by promoting interleukin-7 receptor (IL7R) signaling, and enhances BCR signaling by recruiting PKC to the plasma membrane for substrate phosphorylation (PubMed:28487417). Related references: PMID:28487417
• The protein is involved in lymphocyte homing to lymph nodes by stabilizing L-selectin (SELL) cell surface expression, facilitating B- and T-cell migration.
• CD53 mediates metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF and LPS signaling.
• Subcellularly, CD53 localizes to the cell membrane, cell junctions, and synapses; it is a multi-pass transmembrane glycoprotein expressed primarily in B-cells, monocytes, macrophages, neutrophils, and T-cell subsets.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-180 of human CD53; consider this region when evaluating epitope accessibility in different assay formats.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and optimal working dilutions for WB and ELISA should be determined experimentally in the relevant sample system.
• For western blotting under reducing conditions, a band at approximately 24 kDa is expected; inclusion of positive and negative cell lysate controls (e.g., human peripheral blood mononuclear cells vs. CD53-deficient cells) is recommended.
• In ELISA, antigen coating concentration and detection antibody dilution may require optimization; cross-reactivity with mouse samples should be validated if working with murine models.
• Because CD53 is expressed in multiple immune cell types and hepatocytes, tissue-specific expression patterns should be taken into account when designing experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials, technical services, and consulting—from concept to clinic. As your trusted sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. The CD53 antibody supports research in immune cell signaling and hematological studies, and CamelBio ensures reliable, timely procurement to accelerate your projects.