Anti-CD52 Polyclonal Antibody for WB - P31358

Core Product Specifications and Parameters

Biological Background: CD52 Function and Localization

• CD52 (CAMPATH-1 antigen, CDw52) is a glycoprotein that is linked to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor.
• It is also known as human epididymis-specific protein 5 (HE5) and epididymal secretory protein E5, hinting at a role in the male reproductive tract.
• The mature CD52 protein is small, with a predicted molecular weight of approximately 7 kDa (UniProt lists 6614 Da), after cleavage of the N-terminal signal peptide.
• CD52 is heavily N-glycosylated, and its carbohydrate moieties are thought to contribute to its suggested role in carrying and orienting carbohydrates on the cell surface.
• The protein is localized to the outer leaflet of the cell membrane, tethered by its C-terminal GPI anchor.
• The 3D structure of CD52 has been determined, and its sequence has been verified by direct protein sequencing, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-61) of human CD52, which includes the signal peptide and early mature sequence; this design favors broad epitope recognition.
• For western blotting, consider using a high-percentage SDS-PAGE gel (e.g., 15%) and a PVDF membrane with small pore size to efficiently transfer the small 7 kDa protein.
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval and blocking steps for membrane antigens; validate in the relevant tissue model using appropriate controls.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes, offering robust signal but potential batch-to-batch variability; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials like this CD52 polyclonal antibody, supporting immunology and glycol-related biomarker development. We offer one-stop access to validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare or custom targets. Let CamelBio be your reliable partner from concept to clinic, ensuring streamlined procurement for your CD52 research and diagnostic needs.