Anti-CD50 (ICAM-3) Monoclonal Antibody for Flow Cytometry - P32942

Core Product Specifications and Parameters

Biological Background: CD50 (ICAM-3) Function and Localization

• Protein identity: Intercellular adhesion molecule 3 (ICAM-3), also known as CD50 or CDw50, is a member of the immunoglobulin superfamily and functions as a cell adhesion molecule.
• LFA-1 ligand and phagocytosis: ICAM-3 acts as a ligand for the integrin LFA-1 (ITGAL:ITGB2), mediating leukocyte adhesion and facilitating apoptotic neutrophil phagocytosis by macrophages. Related references: PMID:1448173 PMID:1448174 PMID:23775590
• Additional integrin interactions: It also serves as a ligand for integrin ITGAD/ITGB2, expanding its role in leukocyte adhesion events. Related references: PMID:8777714
• DC-T cell interaction: ICAM-3 binds to CD209 (DC-SIGN) on dendritic cells in a Ca²⁺-dependent manner, establishing initial contact with resting T cells and promoting DC-induced T cell proliferation. Related references: PMID:10721994
• Subcellular localization: ICAM-3 is a single-pass type I membrane protein located on the cell membrane.
• Tissue specificity: It is constitutively expressed exclusively on resting leukocytes and is absent on resting or cytokine-activated endothelial cells. Related references: PMID:8325327 PMID:1448174
• Structural features: ICAM-3 contains immunoglobulin-like domains, disulfide bonds, and is extensively glycosylated. It undergoes phosphorylation and has been identified through proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30-114 in the N-terminal extracellular region, suggesting the antibody likely recognizes an epitope within this domain.
• This rabbit monoclonal, conjugated to , is intended for flow cytometry applications. Consider using it for detection of CD50 on live, non-permeabilized leukocytes.
• Validate the antibody in the relevant sample system, such as human peripheral blood mononuclear cells (PBMCs) or leukocyte cell lines, and include appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material supply. This CD50 (ICAM-3) antibody supports research into leukocyte adhesion, immune cell interactions, and related diagnostic development. Whatever your procurement needs—from concept to clinic—CamelBio streamlines access to high-quality raw materials and technical consulting for the IVD industry.