Anti-CD5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5, also referred to as T1 or Leu-1, is a lymphoid-specific transmembrane receptor characterized by three extracellular scavenger receptor cysteine-rich (SRCR) domains, a single transmembrane helix, and a cytoplasmic tail involved in signal transduction.
• It is predominantly expressed on all T-cells and a subset of B1a B-cells, playing a crucial role in modulating T-cell receptor (TCR) and B-cell receptor (BCR) signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance.
• CD5 serves as a negative regulator of TCR signaling by interacting with key signaling molecules such as LCK, CD3ζ, PI3K, and CBL. Upon co-engagement of CD3, phosphorylation of CBL is enhanced, which promotes VAV1 phosphorylation and subsequent degradation. Related references: PMID:1384049 PMID:1385158 PMID:23376399
• Through interactions with ligands like CD5L and CD72 on B-cells, CD5 facilitates contact-mediated, T-dependent B-cell activation and helps maintain regulatory T and B-cell homeostasis.
• In B-cells, CD5 signaling leads to ERK1/2 activation via the calcium channel TRPC1, resulting in IL-10 production and modulation of B-cell effector functions. Related references: PMID:27499044
• The CD5 protein localizes to the cell membrane and undergoes post-translational modifications including N-linked glycosylation and phosphorylation, which regulate its stability and signaling activity.
• Keywords associated with CD5 include receptor, immune response, disulfide bond, glycoprotein, and phosphoprotein, underscoring its multifaceted role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody corresponds to amino acids 158–371 of human CD5, which spans a portion of the extracellular domain. This design may favor detection of surface CD5 in immunohistochemistry (IHC-P) applications.
• For Western blot (WB), a band at approximately 55 kDa is expected. Optimization of sample preparation, loading amount, and antibody dilution (e.g., 1:500–1:2000) is recommended using positive control cell lines such as Jurkat (human T-cell leukemia) or mouse splenocytes.
• In IHC-P, antigen retrieval method and antibody titration should be empirically determined. Cross-reactivity with mouse and rat CD5 allows the antibody to be used in common rodent model systems.
• For ELISA, the antibody may be used as a capture or detection reagent. Consider pairing with a matched antibody pair if quantitative analysis is required. Validate matrix effects and specificity using recombinant CD5 protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, technical services, and expert consultation. This CD5 polyclonal antibody is suited for research into T-cell biology, autoimmune diseases, and B-cell malignancies. Whether you require bulk antibody supply, custom antibody development, or assistance with rare target sourcing, CamelBio offers a streamlined procurement pathway to keep your projects moving forward.