Anti-CD5 Monoclonal Antibody for Flow Cytometry - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and in a subset of B-cells known as B1a cells.
• It plays a critical role in regulating TCR and BCR signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance.
• CD5 interacts with ligands such as CD5L and CD72 on B-cells, contributing to contact-mediated T-dependent B-cell activation and maintenance of regulatory T and B-cell homeostasis.
• It functions as a negative regulator of TCR signaling during thymocyte development by associating with signaling proteins including LCK, CD3Z chain, PI3K, and CBL. Related references: PMID:1384049 PMID:1385158
• Co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment, leading to increased VAV1 phosphorylation and degradation. Related references: PMID:23376399
• CD5 modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via TRPC1, leading to IL-10 production. Related references: PMID:27499044
• It is a transmembrane glycoprotein located at the cell membrane, featuring a disulfide bond, phosphorylation sites, and a resolved 3D-structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25-371) of human CD5, making this antibody suitable for detecting surface CD5 expression by flow cytometry.
• For successful flow cytometry staining, titrate the antibody to determine the optimal working concentration for your specific cell type and experimental setup.
• Include appropriate isotype controls and unstained controls to accurately gate CD5-positive populations, particularly in human PBMCs or T-cell lines.
• Consider validating the antibody's reactivity with your target cell subset, noting that CD5 is expressed on all T-cells and B1a B-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated sourcing hub for IVD raw materials, connecting diagnostic manufacturers and laboratories with high-quality immunological reagents. This anti-human CD5 rabbit monoclonal antibody targets a key T-cell surface marker, supporting immune profiling and clinical flow cytometry applications. Complementing this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing to streamline your assay development and manufacturing needs.