Anti-CD48 Rabbit Monoclonal Antibody for WB, FC, ELISA - P09326

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48 (SLAMF2) is a member of the signaling lymphocytic activation molecule (SLAM) family, encoded by the CD48 gene; alternative splicing generates multiple isoforms.
• As a GPI-anchored cell surface glycoprotein, CD48 interacts with CD2 and CD244/2B4 to regulate immune cell activation and adhesion. Related references: PMID:12007789, PMID:19494291, PMID:27249817
• CD48 engagement facilitates T-cell receptor signaling by recruiting the Src kinase LCK and the adaptor LAT to the CD3 complex, thereby promoting LCK phosphorylation and downstream activation. Related references: PMID:19494291, PMID:12007789
• In NK cells and cytotoxic T lymphocytes, CD48 binding induces phosphorylation of CD244 ITSMs, triggering immunological synapse formation and directed exocytosis of cytolytic granules containing perforin and granzymes. Related references: PMID:27249817
• The protein localizes primarily to the cell membrane and membrane rafts, with a soluble secreted form also detected.
• CD48 is widely expressed on all hematopoietic cells, underscoring its central role in immune regulation.
• Key post-translational modifications include N-linked glycosylation, disulfide bond formation, and the attachment of a GPI anchor.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 27–220) of human CD48; consider this when designing assays for surface or soluble target detection.
• In Western blot, bands around 19 kDa and 27 kDa are commonly observed, likely reflecting differential glycosylation; optimize sample preparation conditions accordingly.
• Suitable for flow cytometry and ELISA; titrate the antibody and validate in your specific sample system (e.g., PBMCs, cell lines).
• Cross-reactivity with mouse CD48 is reported; verify reactivity in mouse tissues or cells if cross-species applications are planned.
• For flow cytometry, standard surface staining protocols are recommended; no permeabilization is required for membrane-bound CD48.

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