Anti-CD48 Rabbit Monoclonal Antibody for Flow Cytometry and ELISA - P18181

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48, also known as SLAMF2 or BLAST-1, is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein belonging to the signaling lymphocytic activation molecule (SLAM) family.
• It modulates immune responses by engaging the receptors CD2 and 2B4/CD244, which are critical for T-cell and natural killer (NK) cell activation (PubMed:9881969, PubMed:21278219). Related references: PMID:9881969, PMID:21278219
• Upon CD2 ligation, CD48 recruits the Src kinase LCK and the linker LAT to the TCR/CD3 complex, thereby enhancing tyrosine phosphorylation and downstream signaling cascades (PubMed:9881969). Related references: PMID:9881969
• Interaction with 2B4/CD244 triggers phosphorylation of immunoreceptor tyrosine-based switch motifs (ITSMs), leading to immunological synapse assembly and polarized exocytosis of cytotoxic granules by effector lymphocytes (PubMed:21278219). Related references: PMID:21278219
• CD48 is predominantly located in lipid rafts of the plasma membrane and can also be released in a soluble form; its GPI anchor facilitates compartmentalization for efficient signal transduction.
• Mature CD48 has a molecular weight of ~27 kDa (UniProt: 27383 Da) and contains immunoglobulin-like domains, disulfide bonds, and N-linked glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 23–216) encompasses the extracellular domain of mouse CD48, which may be suitable for detecting native surface-exposed epitopes in flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal working concentration and include appropriate isotype controls to assess non-specific binding.
• In ELISA applications, pairing with a capture antibody or using recombinant CD48 protein as a standard could help develop quantitative detection systems.
• Take care to preserve membrane integrity during sample preparation if targeting membrane-bound CD48, as its GPI anchor may be sensitive to certain detergents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers. Our expertise in rare target procurement supports the development of immunoassays for immune cell surface markers such as CD48, streamlining your path from concept to clinical application.