Anti-CD47 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q08722

Core Product Specifications and Parameters

Biological Background: Leukocyte Surface Antigen CD47 Function and Localization

• CD47, also known as IAP (Integrin-Associated Protein), OA3, or MER6, is a broadly expressed transmembrane glycoprotein of the immunoglobulin superfamily.
• Functions as an adhesive protein and receptor for thrombospondin-1 (THBS1), modulating integrin signaling through heterotrimeric G proteins (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:7691831, PubMed:8550562).
  Related references: PMID:11509594, PMID:15383453, PMID:19004835, PMID:7691831, PMID:8550562
• Plays a central role in signal transduction, inflammation, apoptosis, angiogenesis, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835).
  Related references: PMID:11509594, PMID:15383453, PMID:19004835
• Serves as a receptor for SIRPA, inhibiting dendritic cell maturation and cytokine production (PubMed:11509594); interacts with SIRPG to mediate cell-cell adhesion and T-cell activation (PubMed:15383453).
  Related references: PMID:11509594, PMID:15383453
• Modulates nitric oxide (NO) signaling and supports blood pressure regulation in response to THBS1 (By similarity).
• Important for memory formation and synaptic plasticity in the hippocampus (By similarity).
• Localizes to the cell membrane, consistent with its role in cell adhesion and signal transduction.
• Broadly expressed in normal adult tissues, with abundant levels in epithelia, brain, and macrophages (PubMed:39121194).
  Related references: PMID:39121194
• Subject to glycosylation, disulfide bond formation, phosphorylation, and pyrrolidone carboxylic acid modification, reflecting its complex post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved cytoplasmic region (amino acids 224–323) of human CD47; cross-reactivity with mouse and rat is reported, but validation in the target species is advised.
• For Western blotting, a band at approximately 35 kDa is expected; however, due to glycosylation, the observed molecular weight may be higher in certain sample types. Optimize reducing conditions and run appropriate molecular weight markers.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval techniques and appropriate blocking to minimize background.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls in each assay.
• Suitable for ELISA applications; ensure optimal coating conditions and antibody titrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline procurement of high-quality IVD raw materials. For CD47-related research in oncology and immunology, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our team also supports rare target sourcing and technical consultation, bridging the gap from concept to clinic.