Anti-CD47 Rabbit Polyclonal Antibody for WB, ELISA - Q61735

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• CD47, also known as integrin-associated protein (IAP), is a ubiquitously expressed transmembrane glycoprotein of the immunoglobulin superfamily.
• It functions as a receptor for thrombospondin-1 (THBS1), modulating integrin signaling and heterotrimeric G protein activation, and plays a key role in cardiovascular homeostasis, nitric oxide signaling, and blood pressure regulation. Related references: PMID:20610415 PMID:27742621
• Through SIRPA binding, it transmits an anti-phagocytic signal that protects red blood cells from elimination, and by similarity, suppresses dendritic cell activation. Related references: PMID:10856220
• Positively regulates FAS-dependent apoptosis in T-cells by facilitating FAS clustering. Related references: PMID:15917238
• Inhibits stem cell self-renewal and negatively modulates wound healing downstream of THBS1, involving regulation of pluripotency transcription factors POU5F1/OCT4, SOX2, MYC, and KLF4. Related references: PMID:23591719 PMID:18156939
• Suppresses angiogenesis and has been implicated in metabolic dysregulation during normal aging. Related references: PMID:32679764
• Primarily localized to the cell membrane, with an extracellular immunoglobulin V-type domain, multiple transmembrane helices, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation, phosphorylation, an N-terminal pyrrolidone carboxylic acid, and disulfide bonds, which modulate its structure and ligand interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 19–135), which contains the ligand-binding immunoglobulin domain. Epitope preservation in native conformation is critical; users may evaluate the antibody in non-reducing SDS-PAGE for disulfide bond-dependent epitopes.
• For Western blotting, a band around 33 kDa is expected; note that glycosylation may cause the protein to migrate at a slightly higher molecular weight.
• The antibody is validated for detection of mouse and rat CD47; cross-reactivity with other species should be independently verified.
• For ELISA, consider pairing with a detection antibody or using the antibody for direct/indirect detection, and optimize coating conditions for recombinant or native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a wide portfolio of validated antibodies, antibody pairs, and ancillary reagents to accelerate diagnostic development. This rabbit polyclonal anti-CD47 antibody supports research in immuno-oncology, cardiovascular biology, and stem cell biology. Contact us for bulk quantities, custom antibody services, or to source complementary reagents for your CD47-targeted assay.