Anti-CD45RO Monoclonal Antibody () for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45RO Function and Localization

• CD45RO is a splice variant of the PTPRC gene, encoding the leukocyte common antigen (CD45), a receptor-type tyrosine-protein phosphatase.
• CD45 is essential for T-cell activation through the antigen receptor, acting as a positive regulator of coactivation upon DPP4 binding. Related references: PMID:35767951
• The first phosphatase domain is enzymatically active, while the second modulates substrate specificity, enabling fine-tuned regulation of signaling.
• Dephosphorylates key signaling molecules such as SKAP1, FYN, and LYN, thereby modulating T-cell receptor signaling and activation threshold.
• Localizes to the cell membrane, membrane rafts, and the immunological synapse, where it facilitates antigen recognition and signaling.
• Multiple isoforms, including those detected in thymocytes, are generated by alternative splicing; CD45RO is predominantly associated with memory T cells.
• Functions as a receptor for human cytomegalovirus protein UL11, leading to impaired T-cell proliferation and reduced tyrosine phosphorylation upon T-cell receptor stimulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human CD45RO; verify that the antibody recognizes native CD45RO in your target cell type under your experimental conditions.
• Flow cytometry is the validated application; titrate the antibody to determine the optimal dilution for your specific panel and instrument settings.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and detection of CD45RO-positive populations.
• For multicolor flow cytometry, consider potential spectral overlap of with other fluorophores in the same channel (e.g., FITC, Alexa Fluor 488).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CD45RO product for immunophenotyping applications. Beyond single reagents, we offer optimized antibody pairs, bulk ancillary reagents, and sourcing services for rare targets, ensuring a streamlined supply chain from concept to clinical validation. Partner with us to accelerate your T-cell biology and flow cytometry assay development with reliable, research-grade materials.