Anti-CD45RB Monoclonal Antibody for WB - P08575-9

Core Product Specifications and Parameters

Biological Background: CD45RB Function and Localization

• CD45RB is an isoform of receptor-type tyrosine-protein phosphatase C (PTPRC), a member of the protein tyrosine phosphatase family.
• Commonly referred to as Leukocyte common antigen (L-CA) or T200.
• Encoded by the PTPRC gene (CD45) in humans.
• Isoform 7 is predicted to have a molecular weight of approximately 135.7 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider using a 7-10% SDS-PAGE gel due to the predicted 135 kDa protein size.
• In flow cytometry, CD45RB expression is typically observed on various leukocyte subsets; validate gating strategies in your cell populations of interest.
• The immunogen spans a large N-terminal extracellular domain (amino acids 26-463), which may facilitate detection in IHC-P and IF applications.
• For IHC-P, appropriate antigen retrieval methods should be optimized for your tissue samples.
• Perform standard blocking and antibody titration for each application in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your CD45RB-targeted immunoassay development. Leverage our one-stop sourcing solution for rare and specialized targets to accelerate your assay development from concept to clinic.