Anti-CD45 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, ELISA - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (receptor-type tyrosine-protein phosphatase C), also known as leukocyte common antigen (L-CA), LY5, B220, and T200, is encoded by the PTPRC gene. Multiple isoforms arise from alternative splicing.
• It is a critical positive regulator of T-cell activation through the antigen receptor. The first phosphatase domain has catalytic activity, while the second modulates substrate specificity. Related references: PMID:35767951
• CD45 dephosphorylates key signaling molecules: upon T-cell activation, it recruits and dephosphorylates SKAP1 and FYN, and modulates LYN activity. Interaction with CLEC10A at the dendritic cell–T cell interface modulates the activation threshold to limit autoreactivity.
• It acts as a receptor for human cytomegalovirus protein UL11, mediating binding to T-cells and impairing TCR signaling and proliferation.
• Subcellularly, CD45 localizes to the cell membrane, membrane rafts, and immunological synapse. It is extensively glycosylated and phosphorylated.
• Tissue-specific isoform expression: isoforms 1, 2, 3, 5, and 7 are detected in thymocytes, while isoforms 4, 6, and 8 are not.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at ~147 kDa is expected; validate the antibody in appropriate leukocyte lysates from human, mouse, or rat.
• In immunoprecipitation (IP), CD45 enrichment can be achieved; co-immunoprecipitation experiments may require optimization of lysis and washing conditions.
• For immunofluorescence (IF/ICC and IF-P), fixation and permeabilization protocols should be optimized; CD45 surface staining can be performed without permeabilization.
• The immunogen (aa 611–730) lies within the extracellular region, suggesting potential suitability for live-cell surface staining, though validation is recommended.
• Reported cross-reactivity with mouse and rat makes this antibody a candidate for multi-species studies; always verify specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with seamless access to high-quality IVD raw materials. For CD45 research—spanning immunophenotyping, leukemia diagnostics, and immunotherapy—we offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Contact us to streamline your supply chain and accelerate development from concept to clinic.