Anti-CD45 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (Receptor-type tyrosine-protein phosphatase C, PTPRC) is a transmembrane phosphatase essential for T-cell receptor signaling and lymphocyte activation.
• The protein phosphatase domain dephosphorylates key signaling molecules such as LYN, FYN, and SKAP1, modulating T-cell coactivation upon binding to DPP4 (PubMed:35767951). Related references: PMID:35767951
• CD45 exists as multiple isoforms generated by alternative splicing; isoform expression is tissue-specific and varies among thymocyte subsets.
• It localizes to the cell membrane, membrane rafts, and immunological synapse, where it facilitates antigen-presenting cell–T cell interactions.
• Acts as a receptor for human cytomegalovirus protein UL11, leading to impaired T-cell proliferation and reduced tyrosine phosphorylation during infection.
• Annotated with keywords including glycoprotein, host-virus interaction, and disease variant, highlighting its role in immune processes and pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 194–390 within the extracellular domain, which may preferentially detect certain CD45 isoforms; validate specificity in your experimental system.
• For Western blotting, consider using a low percentage gel (e.g., 6–8%) due to the high molecular weight (~147 kDa) and ensure sufficient transfer time.
• In IHC-P, antigen retrieval method and antibody dilution should be optimized for your tissue type; strong membrane staining is expected in leukocytes.
• ELISA performance may depend on plate coating and detection format; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD45-related research—a cornerstone of leukocyte biology and diagnostic immunology—we facilitate one-stop access to high-quality antibodies and rare target reagents, supporting your workflow from concept to clinic.