Anti-CD45 Rabbit Monoclonal Antibody for IF/ICC, IHC-P, FC, ELISA - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as Leukocyte common antigen (L-CA), Ly-5, or T200, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene in mice. It is a transmembrane glycoprotein with a molecular weight of approximately 145 kDa.
• CD45 is essential for T-cell activation through the antigen receptor. It functions as a positive regulator of T-cell coactivation upon binding to DPP4.
• The intracellular region contains two tandem protein tyrosine phosphatase (PTP) domains. The first domain possesses catalytic activity, while the second domain modulates substrate specificity.
• Upon T-cell activation, CD45 recruits and dephosphorylates signaling molecules such as SKAP1 and FYN, and also dephosphorylates LYN to modulate its activity.
• CD45 interacts with CLEC10A at the contact site between antigen-presenting cells and T cells. This interaction, involving Tn antigen-carrying CD45 on effector T cells and CLEC10A on immature dendritic cells, helps tune T-cell activation thresholds to prevent autoreactivity.
• The protein localizes to the cell membrane, membrane rafts, and immunological synapses, consistent with its role in proximal signaling. Post-translational modifications include alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26–566 of mouse CD45 (the extracellular region), suggesting the antibody likely recognizes an extracellular epitope. This makes it well-suited for live-cell staining and flow cytometry without permeabilization.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), consider optimizing fixation conditions (e.g., 4% PFA) and, if needed, use mild permeabilization to ensure antibody access while preserving epitope integrity.
• When performing flow cytometry (FC), titrate the antibody to determine the optimal concentration for your specific mouse cell type, and include appropriate isotype controls.
• This antibody is validated for use with mouse samples. Cross-reactivity with other species has not been reported; verify in your system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing access to a broad portfolio of IVD raw materials, including monoclonal antibodies like this anti-CD45 clone for immunophenotyping and T-cell research. Our supply chain covers validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials, streamlining your development from concept to clinical application.