Anti-CD45 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (PTPRC) is a receptor-type tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Related references: PMID:35767951
• It acts as a positive regulator of T-cell coactivation upon binding to DPP4, and dephosphorylates substrates such as SKAP1, FYN, and LYN to modulate downstream signaling.
• CD45 localizes to the cell membrane, membrane rafts, and the immunological synapse, positioning it for dynamic regulation of immune cell communication.
• Alternative splicing generates multiple isoforms; isoforms 1, 2, 3, 5, and 7 are detected in thymocytes, while isoforms 4, 6, and 8 are not, suggesting tissue-specific roles.
• In microbial infection, CD45 functions as a receptor for human cytomegalovirus protein UL11, mediating binding to T-cells and impairing T-cell receptor-induced signaling and proliferation.
• Keywords from UniProt include glycoprotein, alternative splicing, disease variant, host-virus interaction, phosphoprotein, and SCID, highlighting its central role in immune regulation and pathology.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 194–390, a region within the extracellular domain; consider that the antibody may recognize a linear epitope within this segment.
• For western blot, the predicted molecular weight of CD45 is approximately 147 kDa; extensive glycosylation may cause broader bands or slight migration shifts.
• When performing immunohistochemistry or immunofluorescence, validate the antibody in positive control tissues rich in leukocytes (e.g., thymus, spleen, lymph node).
• The antibody’s cross-reactivity with human, mouse, and rat makes it suitable for comparative studies, but always verify species-specific reactivity in your experimental system.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider including appropriate positive and negative controls in each assay and titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a seamless supply channel for IVD raw materials, including validated polyclonal antibodies like this anti-CD45 pAb. Our portfolio spans optimized monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate T-cell and leukocyte research. For rare or specialized targets, we provide sourcing and technical consultation to bridge your concept-to-clinic development needs.