Anti-CD45 Monoclonal Antibody for WB, IHC-P, mIHC, ELISA - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, officially designated receptor-type tyrosine-protein phosphatase C (PTPRC), is also known as leukocyte common antigen (L-CA), T200, and B220 (in its isoform-specific context). It is a key transmembrane glycoprotein expressed on all nucleated hematopoietic cells.
• This phosphatase is essential for T-cell activation and acts as a positive regulator of T-cell co-activation upon binding to DPP4. Its first phosphatase domain possesses enzymatic activity, while the second modulates substrate specificity. Upon T-cell activation, CD45 recruits and dephosphorylates signaling molecules such as SKAP1, FYN, and LYN. Related references: PMID:35767951
• In the context of microbial infection, CD45 functions as a receptor for human cytomegalovirus protein UL11, mediating UL11 binding to T-cells. This interaction leads to reduced tyrosine phosphorylation of signaling proteins upon TCR stimulation and impaired T-cell proliferation.
• Subcellularly, CD45 localizes to the cell membrane, with enrichment in membrane rafts and at the immunological synapse, underscoring its role in proximal signaling events.
• The gene PTPRC undergoes extensive alternative splicing, giving rise to multiple isoforms (e.g., isoforms 1–8) that exhibit distinct tissue-specific expression patterns, particularly in thymocytes.
• Post-translational modifications include N-glycosylation and phosphorylation, and mutations in PTPRC are linked to severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• For Western blot, account for the large molecular weight (~147 kDa) by using an appropriate low-percentage SDS-PAGE gel and extended transfer conditions. Include a positive control lysate from a human leukocyte cell line (e.g., Jurkat) to verify band specificity.
• In IHC-P and mIHC, optimize antigen retrieval (e.g., heat-induced epitope retrieval) and titrate the antibody concentration, as the reactivity is human-specific. Counterstain with a nuclear dye to visualize cell morphology.
• For ELISA, this monoclonal antibody can serve as a detection reagent; consider pairing with a capture antibody that recognizes a non-overlapping CD45 epitope if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to premium IVD raw materials, including this high-quality rabbit monoclonal antibody against human CD45. As a one-stop sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source even rare-target raw materials to accelerate your assay development—from concept to clinic.