Anti-CD44 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16070

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a cell-surface receptor that binds hyaluronan and other extracellular matrix components, mediating cell adhesion, migration, and microenvironment sensing (PubMed:16541107, PubMed:19703720, PubMed:22726066). Related references: PMID:16541107 PMID:19703720 PMID:22726066
• It plays critical roles in immune cell functions, including T‑lymphocyte activation, recirculation, and homing, as well as hematopoiesis and inflammation (PubMed:7528188). Related references: PMID:7528188
• Through its cytoplasmic domain, CD44 assembles signaling complexes with receptor kinases and membrane proteases, engaging effectors such as PKN2, RAC1, and RHOA to regulate actin‑mediated cytoskeletal reorganization and cell migration (PubMed:18757307, PubMed:23589287, PubMed:15123640). Related references: PMID:18757307 PMID:23589287 PMID:15123640
• Isoform 10 (epithelial isoform) is predominantly expressed in epithelial cells and is highly upregulated in many carcinomas, while its expression is repressed in neuroblastoma cells.
• CD44 localizes to the cell membrane, microvilli, and is also found in a secreted form.
• As a heavily post‑translationally modified protein, CD44 exists as a proteoglycan and phosphoprotein with multiple disulfide bonds and glycosylation sites.
• (Microbial infection) CD44 also promotes foot‑and‑mouth disease virus internalization via macropinocytosis.

Experimental Guidance and Technical Tips

• The immunogen spans residues 20–178 in the N‑terminal extracellular domain; epitopes are expected to be accessible on native and denatured CD44, suitable for applications such as WB, IHC‑P, IF/ICC, and ELISA.
• For Western blotting, the predicted molecular weight is approximately 80 kDa, but extensive glycosylation and alternative splicing may yield additional bands; validate with appropriate positive controls and optimized reducing/non‑reducing conditions.
• In IHC‑P, optimize antigen retrieval (e.g., citrate buffer pH 6.0) and blocking steps; consider using a HRP‑polymer detection system for higher sensitivity.
• For IF/ICC, permeabilization is required if the epitope is intracellular; confirm antibody specificity with knockout or knockdown controls.
• Cross‑reactivity with other species has not been tested; if used on non‑human samples, experimental validation is strongly recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a broad portfolio of validated antibodies, including monoclonal and polyclonal options, for research and diagnostic development. We cater to projects involving CD44—a key player in cell adhesion, cancer, and immune cell trafficking—by supplying high‑quality antibody pairs, bulk ancillary reagents, and rare target raw materials. From concept to clinic, our streamlined procurement and technical support help accelerate your assay development pipeline.