Anti-CD44 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a highly versatile transmembrane glycoprotein known by alternate names including Ly-24, Pgp-1, HERMES, ECMR-III, and hyaluronate receptor.
• It functions as a cell-surface receptor mediating cell adhesion, migration, and T-lymphocyte homing by binding hyaluronan, collagen, and other extracellular matrix components. Ligand engagement assembles cytoplasmic signaling complexes that regulate hematopoiesis, inflammation, and immune cell trafficking. Related references: PMID:25065622 PMID:8343954
• CD44 localizes to the plasma membrane and microvilli and is also found as a secreted form; in mouse, it is notably expressed in the intestinal epithelium.
• The receptor undergoes extensive post-translational modification including N- and O-glycosylation, sulfation, phosphorylation, and proteoglycan substitution, and contains structural disulfide bonds.
• Upon interaction with the LGALS9 ligand, CD44 activates downstream signaling via LCK, ERK, and MAPK to promote natural killer cell activation. Related references: PMID:37006235
• It additionally participates in the response to bacterial infection, reflecting its broader role in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-176) of mouse CD44. Antibodies generated against this region are expected to recognize the native, surface-exposed receptor.
• For Western blotting, a band around 86 kDa is predicted; however, extensive glycosylation may cause migration at a higher apparent molecular weight—include proper positive controls and consider deglycosylation if necessary.
• In immunofluorescence/immunocytochemistry, the antibody should detect membrane-localized CD44; optimize fixation and permeabilization steps to preserve epitope accessibility.
• For flow cytometry, use standard surface staining protocols and titrate the antibody on the relevant mouse cell population.
• The antibody is validated for mouse samples; cross-reactivity with other species has not been determined and should be tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD manufacturers, academic laboratories, and research institutes, covering raw materials, technical consultation, and development support from concept to clinic. This anti-CD44 rabbit monoclonal antibody serves as a high-quality tool for research into immune cell homing, cell adhesion, and cancer biology. We supply validated antibody pairs, bulk ancillary reagents, and customized rare target raw-material sourcing to accelerate your diagnostic assay development.