Anti-CD44 Polyclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC, ELISA - P16070

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a cell-surface receptor that plays a central role in cell-cell interactions, cell adhesion and migration, enabling cells to sense and respond to changes in the tissue microenvironment. Related references: PMID:16541107, PMID:19703720, PMID:22726066
• As the primary receptor for hyaluronan (HA), CD44 also binds collagen, growth factors, cytokines, and proteases, thereby mediating extracellular matrix attachment and signaling.
• CD44 participates in diverse physiological processes including lymphocyte activation, recirculation, homing, hematopoiesis, inflammation, and response to bacterial infection. Related references: PMID:7528188
• Through its cytoplasmic domain, CD44 assembles protein complexes containing receptor kinases and membrane proteases, linking to downstream effectors such as PKN2, RAC1, RHOA, Rho-kinases, and phospholipase C to regulate calcium mobilization and actin cytoskeleton reorganization. Related references: PMID:18757307, PMID:23589287, PMID:15123640
• CD44 interaction with LGALS9 activates NK cells via LCK, ERK, and MAPK pathways. Related references: PMID:37006235
• CD44 exists in multiple isoforms generated by alternative splicing; isoform 10 (epithelial isoform) is highly expressed in carcinomas and is a marker of cancer progression.
• Subcellularly, CD44 localizes to the cell membrane, microvilli, and can be secreted; it is widely detected in fibroblasts, urine, and placenta, with isoform 10 expression restricted to epithelial cells.
• In microbial infection, CD44 promotes viral internalization, as demonstrated for foot-and-mouth disease virus via macropinocytosis.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal ectodomain (aa 20–178) of human CD44; consider sequence alignment with target species to confirm epitope conservation when extending to non-validated species.
• For western blotting, note that CD44 exhibits extensive glycosylation and multiple isoforms; the observed band may differ from the theoretical 82 kDa, so optimize gel percentage and running conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), fixation and antigen retrieval protocols may influence staining; validate in a known positive control tissue such as tonsil or carcinoma samples.
• This polyclonal antibody recognizes both human and mouse CD44; cross-reactivity in other species should be confirmed experimentally.
• For ELISA applications, when pairing with a capture antibody, ensure that the epitope region does not overlap with that of the capture antibody to avoid competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials, technical services, and consulting. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies for critical targets like CD44, or bulk ancillary reagents, our comprehensive supply chain and rare-target sourcing capabilities streamline your development from concept to clinic.