Anti-CD44 Polyclonal Antibody for WB, ELISA - P16070

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 is a cell-surface receptor, also known as PGP-1, ECMR-III, HUTCH-1, and Epican, encoded by the CD44 gene in humans.
• It mediates cell-cell and cell-extracellular matrix adhesion by binding hyaluronan and other ligands, playing a crucial role in lymphocyte homing, hematopoiesis, and inflammation. Related references: PMID:16541107 PMID:19703720 PMID:22726066 PMID:7528188
• Through its cytoplasmic domain, CD44 assembles signaling complexes involving PKN2, RAC1, RHOA, and phospholipase C to regulate calcium mobilization and actin cytoskeletal reorganization. Related references: PMID:15123640
• Alternative splicing generates multiple isoforms; isoform 10 (epithelial isoform) is highly expressed in carcinomas and repressed in neuroblastoma.
• Subcellularly, CD44 localizes to the cell membrane, microvilli, and is also found in a secreted form.
• Post-translational modifications include glycosylation, phosphorylation, and proteoglycan addition; annotated as a glycoprotein, phosphoprotein, and proteoglycan.

Experimental Guidance and Technical Tips

• For Western blot, a band around 82kDa is expected, but alternative splicing and extensive glycosylation may produce multiple bands or a higher apparent molecular weight.
• ELISA applications may be optimized using recombinant CD44 protein as a standard, and the antibody’s reactivity with the immunogen region (amino acids 346–385) should be validated in the relevant sample system.
• Since the antibody cross-reacts with mouse CD44, it can be considered for rodent model studies; however, validate specificity in the target tissue or cell line.
• Secreted forms of CD44 may be present in serum or cell culture supernatants; adjust sample preparation accordingly if detecting soluble CD44.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and laboratories with a reliable supply of CD44 antibodies and related reagents for immunoassay development. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with rare target sourcing—helping you accelerate CD44-based projects in cancer, inflammation, and infectious disease research.