Anti-CD44 Monoclonal Antibody for Flow Cytometry - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• CD44 (Ly-24/Pgp-1) is a cell-surface receptor for hyaluronan and other extracellular matrix components, mediating cell adhesion, migration, and cell-cell interactions.
• It is crucial for lymphocyte homing, hematopoiesis, inflammation, and response to bacterial infection via signaling complexes including PKN2, RAC1, RHOA, and phospholipase C (PubMed:25065622, PubMed:8343954). Related references: PMID:25065622 PMID:8343954
• Upon interaction with LGALS9 ligand, CD44 activates LCK, ERK, and MAPK signaling to promote natural killer cell activation (PubMed:37006235). Related references: PMID:37006235
• Subcellular localization: Cell membrane, microvilli; also secreted as a soluble form.
• Tissue specificity: Expressed in intestinal epithelium.
• Post-translational modifications include glycosylation, phosphorylation, sulfation, and proteolytic processing; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-176) of mouse CD44, suggesting the antibody recognizes an N-terminal epitope, potentially suitable for detecting surface-expressed CD44 on live cells by flow cytometry.
• This -conjugated monoclonal antibody is intended for flow cytometry; no secondary detection reagent is required. Consider optimizing the antibody dilution and using appropriate isotype controls for your specific cell type.
• Due to multiple CD44 isoforms, verify reactivity with your target isoform; knockout or negative cell lines can serve as specificity controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutes. This anti-CD44 antibody supports immunology and inflammation research, and we can source additional CD44-targeting reagents or rare-target raw materials to meet your specific needs. Our services cover every stage from concept to clinic, ensuring streamlined supply and technical support.