Anti-CD42d (GPV) Rabbit Polyclonal Antibody for WB and ELISA - P40197

Core Product Specifications and Parameters

Biological Background: CD42d Function and Localization

• CD42d, also known as platelet glycoprotein V (GPV) or glycoprotein 5, is a component of the GPIb-V-IX complex, which serves as the von Willebrand factor (vWF) receptor on platelets.
• The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to injured vascular surfaces, a critical initiating step in hemostasis and arterial thrombosis.
• CD42d is a single-pass type I membrane protein with a large extracellular domain containing multiple leucine-rich repeats, characteristic of the glycoprotein Ib family.
• It is specifically expressed on platelets and megakaryocytes, consistent with its role in platelet function.
• CD42d is classified under keywords including blood coagulation, cell adhesion, hemostasis, glycoprotein, leucine-rich repeat, membrane, and signal.
• The mature protein has a calculated molecular weight of approximately 61 kDa, consistent with the observed band size in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 17–477) of human CD42d, indicating that the antibody likely targets the native protein on the cell surface; consider this when planning flow cytometry or other surface detection methods, though validation for those applications has not been provided.
• For Western blot analysis, use platelet or megakaryocyte lysates as a positive control; verify the antibody specificity in the relevant sample system.
• The antibody cross-reacts with mouse CD42d, enabling comparative studies between human and mouse platelets; confirm expected bands at ~61 kDa in both species.
• As a polyclonal antibody, potential batch variability should be considered; validate each new lot with appropriate controls to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating platelet biology and hemostasis, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to accelerate your assay development. Contact our team to learn how our integrated supply solutions can support your next project.