Anti-CD42a Polyclonal Antibody for WB, ELISA - P14770

Core Product Specifications and Parameters

Biological Background: CD42a Function and Localization

• Platelet glycoprotein IX (GP9, CD42a) is a component of the GPIb-V-IX complex, which serves as the von Willebrand factor (vWF) receptor on the platelet surface.
• The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to injured vascular walls, a critical initiating event in hemostasis and arterial thrombosis.
• GPIX is thought to possess a membrane insertion and orientation function that facilitates proper complex assembly and surface expression of GPIb.
• The protein is a transmembrane glycoprotein characterized by leucine-rich repeats (LRRs), which are key for protein-protein interactions.
• Mutations in the GP9 gene are associated with Bernard-Soulier syndrome, an autosomal recessive bleeding disorder characterized by giant platelets and thrombocytopenia.
• Subcellular localization is predominantly at the plasma membrane, consistent with its role in platelet adhesion.
• The mature protein has a calculated molecular weight of approximately 19 kDa (19046 Da), and is subject to post-translational modifications including disulfide bond formation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the extracellular domain (amino acids 17–140) of human CD42a. For WB, consider using platelet lysates or cell lines expressing GP9 as positive controls.
• Polyclonal antibodies can exhibit lot-to-lot variability; titrate the antibody and validate in the relevant sample system prior to critical experiments.
• For ELISA, the antibody may be used for direct or indirect detection; consider pairing with a monoclonal anti-GP9 if developing a sandwich assay.
• Cross-reactivity is reported for human; reactivity with other species is not guaranteed and should be validated individually.
• Molecular weight observed in WB may differ from the theoretical 19 kDa due to glycosylation or disulfide bond-induced structural changes; include appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For hemostasis and platelet biology research, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-CD42a, and bulk ancillary reagents tailored to your assay development needs. Our global sourcing network ensures timely delivery of rare targets and custom raw materials, backed by regulatory and technical support to streamline your path from research to clinical application.