Anti-CD41/ITGA2B Polyclonal Antibody for IF/ICC, ELISA - P08514

Core Product Specifications and Parameters

Biological Background: Integrin alpha-IIb Function and Localization

• Integrin alpha-IIb (CD41, GPIIb, platelet membrane glycoprotein IIb) is encoded by the ITGA2B gene.
• It forms the platelet-specific integrin alpha-IIb/beta-3 complex with integrin beta-3 (CD61), serving as a receptor for fibrinogen, fibronectin, vitronectin, and other RGD-containing ligands.
• Upon activation, binding of soluble fibrinogen triggers platelet aggregation, a critical hemostatic and thrombotic mechanism. Related references: PMID:9111081
• The complex also interacts with erythrocyte ICAM4, mediating heterotypic adhesion between erythrocytes and activated platelets. Related references: PMID:12477717
• It localizes to the cell membrane and is predominantly expressed in platelets and megakaryocytes (isoforms 1 and 2), with a distinct isoform 3 found in prostate adenocarcinoma and certain melanoma cell lines. Related references: PMID:2351656, PMID:9809974
• Post‑translational modifications include disulfide bonds, glycosylation, and proteolytic cleavage, which are essential for functional conformation.
• Keywords: calcium‑binding, cell adhesion, disease variant (Glanzmann thrombasthenia), and receptor activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (aa 160–260) of human CD41/ITGA2B, supporting broad reactivity with human isoforms.
• For IF/ICC, consider that the target is a transmembrane cell‑surface protein; mild permeabilization may be used to enhance antibody accessibility if needed.
• In ELISA applications, the antibody can be employed for direct or indirect detection using recombinant or purified protein as the coating antigen.
• Validate the antibody in your specific sample system (e.g., platelets, megakaryocytic cell lines) to confirm reactivity and specificity.
• Note that the predicted molecular weight is ~113 kDa, but glycosylation may lead to a higher apparent molecular weight on blots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and supply‑chain support for diagnostic manufacturers and laboratories. Our offerings for hemostasis and thrombosis research include validated anti‑CD41 antibodies, recombinant proteins, and bulk ancillary reagents. Whether you need assay‑ready antibody pairs or rare‑target raw materials, our streamlined sourcing process helps accelerate your project from concept to clinic.