Anti-CD41/ITGA2B Monoclonal Antibody for WB, IHC-P, ELISA - P08514

Core Product Specifications and Parameters

Biological Background: CD41/ITGA2B Function and Localization

• Integrin alpha-IIb (CD41, GPIIb), encoded by ITGA2B, is a transmembrane glycoprotein that pairs non-covalently with ITGB3 to form the platelet-specific integrin complex αIIbβ3, essential for platelet adhesion and aggregation.
• The αIIbβ3 complex serves as a receptor for multiple ligands, including fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, and vitronectin, typically recognizing RGD sequences.
• Following platelet activation, αIIbβ3 binds soluble fibrinogen, leading to platelet crosslinking and rapid aggregation that physically plugs damaged endothelial surfaces. Related references: PMID:9111081
• ITGA2B:ITGB3 also functions as a receptor for erythrocyte ICAM4, mediating heterotypic adhesion between red blood cells and activated platelets. Related references: PMID:12477717
• Subcellularly, integrin alpha-IIb localizes to the cell membrane, where it participates in outside-in signaling and cytoskeletal linkage.
• Isoforms 1 and 2 are expressed in platelets and megakaryocytes, while isoform 3 is detected in prostate adenocarcinoma and erythroleukemia cell lines but not in normal platelets.
• The protein undergoes extensive post-translational modifications, including cleavage, glycosylation, and disulfide bond formation; its activity requires calcium and other divalent metal cations.
• Mutations in ITGA2B are linked to platelet-type bleeding disorders (BDPLT2, BDPLT16), underscoring its importance in hemostasis.

Experimental Guidance and Technical Tips

• This antibody, raised against an N-terminal peptide (aa 1–100), likely recognizes all CD41/ITGA2B isoforms. For isoform-specific detection, verify epitope conservation in your target isoform.
• In Western blot, CD41/ITGA2B migrates at approximately 113 kDa under reducing conditions; use an appropriate high-molecular-weight marker and validate antibody specificity with a suitable control.
• For IHC-P, antigen retrieval conditions may need optimization; test the antibody on platelet-rich tissues (e.g., spleen, bone marrow) or megakaryocyte-like cell lines.
• In ELISA, this antibody can serve as either a capture or detection reagent; confirm cross-reactivity with your sample matrix and include proper negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. This anti-CD41/ITGA2B rabbit monoclonal antibody supports hemostasis and platelet disorder research, and CamelBio can assist with scalable supply, conjugations, and complementary detection tools to accelerate your diagnostic development from concept to clinic.