Anti-CD40 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), Bp50, or CDw40, is a cell surface receptor predominantly expressed on B cells and primary carcinomas.
• It serves as the receptor for CD40 ligand (TNFSF5/CD40LG), initiating intracellular signaling cascades that involve TRAF6 and MAP3K8, ultimately activating ERK and promoting immunoglobulin secretion. Related references: PMID:31331973
• CD40 is localized primarily to the cell membrane, but an alternatively spliced, secreted isoform has also been identified.
• The protein contains several structural motifs critical for its function, including a signal peptide, a transmembrane helix, disulfide bonds, and glycosylation sites.
• CD40 plays a central role in humoral immunity by mediating B-cell activation, proliferation, and differentiation.
• Variants in the CD40 gene have been associated with disease phenotypes, underscoring its importance in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen for this rabbit polyclonal antibody comprises a recombinant fragment spanning amino acids 198–277 of human CD40, a region that is likely extracellular and membrane-proximal; this design may confer recognition of both native and denatured forms.
• For Western blot applications, use a standard reducing and denaturing sample preparation. Validate in human B-cell lines (e.g., Ramos, Raji) or mouse splenocyte lysates. The predicted band is approximately 31 kDa.
• In immunofluorescence and immunohistochemistry, expect membranous staining pattern. Optimize antigen retrieval for paraffin-embedded tissues and consider appropriate blocking steps to minimize nonspecific background.
• For ELISA, this polyclonal antibody can be employed as either a capture or detection reagent, but pairing with a validated monoclonal antibody may improve assay specificity. It is advisable to titrate the antibody for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to deliver comprehensive IVD raw-material solutions. This CD40 rabbit polyclonal antibody supports immunology and oncology research into B-cell biology and carcinoma biomarker studies. Beyond this product, CamelBio can source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development from concept to clinic.