Anti-CD40 Polyclonal Antibody for WB, IHC-P, ELISA - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 (TNFRSF5) is a member of the tumor necrosis factor receptor superfamily, also known as Bp50 or B-cell surface antigen CD40.
• It functions as a receptor for CD40 ligand (CD40LG), triggering TRAF6- and MAP3K8-mediated ERK activation leading to immunoglobulin secretion in B cells (By similarity). Related references: PMID:31331973
• CD40 is primarily expressed on B lymphocytes and has been detected in primary carcinomas.
• The protein exists as a cell membrane receptor, but a secreted isoform has also been identified.
• Several post-translational modifications are predicted, including glycosylation and disulfide bond formation, which are typical of TNFR family members.
• Alternative splicing generates multiple transcript variants, potentially leading to isoforms with distinct functional properties.
• Disease-associated variants in CD40 have been linked to immunodeficiency syndromes, highlighting its critical role in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 25–104), suggesting the antibody may recognize both membrane-bound and secreted CD40 forms.
• For WB, the predicted core protein weight is 31 kDa, but extensive N-glycosylation often results in observed bands at 45–50 kDa; validate the actual molecular weight in your sample system.
• Given the listed cross-reactivity with human, mouse, and rat, consider performing species-specific validation before use in non-human models.
• For IHC-P, antigen retrieval conditions may be necessary; test a range of pH and heat protocols to optimize staining in FFPE tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner for diagnostic manufacturers and research laboratories, providing a seamless supply chain for IVD raw materials and technical support. For CD40-focused projects in immunology and oncology, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—helping you progress from concept to clinical application with confidence.