Anti-CD4 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a 51 kDa transmembrane glycoprotein and a member of the immunoglobulin superfamily, also known as L3T4 or T-cell surface antigen T4/Leu-3.
• It serves as a coreceptor for MHC class II molecules, interacting with the T-cell receptor (TCR) and the MHC II–peptide complex on antigen-presenting cells.
• CD4 recruits the Src kinase LCK to the TCR-CD3 complex, triggering intracellular signaling pathways that regulate T-cell activation, cytokine production, and proliferation.
• Beyond T cells, CD4 is also expressed on macrophages, dendritic cells, and NK cells, where it influences differentiation, cytokine expression, and cell migration.
• The protein localizes predominantly to the cell membrane and is highly expressed on T-helper cells, which are central to adaptive immunity.
• Key post-translational modifications include N-linked glycosylation, disulfide bond formation, and palmitoylation, which are important for structural integrity and membrane association.
• Alternative splicing yields multiple isoforms with potentially distinct functional roles.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider testing reducing and non-reducing conditions using lysates from mouse or human T-helper cells; the expected band is approximately 51 kDa.
• In immunofluorescence (IF/ICC, IF-P), validate fixation and permeabilization protocols for the specific cell type or tissue, as the immunogen targets the extracellular domain.
• The antibody shows cross-reactivity with human CD4, making it suitable for comparative studies, but independent validation in the target system is recommended.
• Optimal working dilutions should be determined empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing a comprehensive selection of validated antibodies, including this anti-CD4 polyclonal antibody for T-cell and immune function research. Our offerings span optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, all backed by technical consulting to support your development from concept to clinic.