Anti-CD4 Monoclonal Antibody () for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is an integral membrane glycoprotein that serves as a coreceptor for MHC class II molecules, playing a central role in T-cell activation and immune responses.
• Upon TCR engagement, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote lymphokine production, adhesion, and T-helper cell differentiation.
• CD4 is a primary receptor for HIV-1, facilitating viral entry; it is also a receptor for human herpesvirus 7 (HHV-7). Related references: PMID:12089508, PMID:16331979, PMID:2214026, PMID:17346169, PMID:7909607
• In the thymus, CD4 participates in T-helper cell development, and it also triggers monocyte-to-macrophage differentiation.
• CD4 is highly expressed on T-helper cells and is also present on macrophages, dendritic cells, and NK cells.
• The protein is localized to the cell membrane and contains extracellular immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail subject to palmitoylation and phosphorylation.

Experimental Guidance and Technical Tips

• Because the conjugate is similar in spectral properties to FITC/Alexa Fluor 488, it is compatible with standard 488 nm laser lines for flow cytometry.
• Given that the immunogen spans the extracellular domain (aa 26–207), the antibody is likely to recognize an extracellular epitope, making it suitable for live-cell staining without permeabilization.
• Consider titrating the antibody to determine the optimal staining concentration that provides the best signal-to-noise ratio for your specific sample type.
• Include appropriate positive and negative controls, such as human peripheral blood mononuclear cells (PBMCs) or known CD4+ and CD4- cell lines, to confirm specificity.
• Avoid prolonged exposure to light to maintain fluorophore stability; store the antibody at 2–8 °C protected from light for short-term use, and aliquot and freeze at ‑20 °C for long-term storage.
• As a monoclonal antibody, it offers consistent lot-to-lot performance and minimal background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research institutes, and clinical laboratories from early development to commercial scale. This anti-CD4 monoclonal antibody is part of a broad portfolio of immune cell surface markers that enable flow cytometry-based immunophenotyping. CamelBio can also supply validated antibody pairs, bulk ancillary reagents, and assist with rare target material sourcing, helping you streamline your T-cell research or diagnostic assay development.