Anti-CD3H Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P20963

Core Product Specifications and Parameters

Biological Background: CD3H Function and Localization

• CD3H (CD247/CD3Z) is the zeta chain of the T-cell receptor (TCR)-CD3 complex, essential for adaptive immune responses and T-cell development.
• Phosphorylation of its immunoreceptor tyrosine-based activation motifs (ITAMs) by LCK/FYN upon TCR engagement recruits and activates ZAP70, initiating downstream signaling cascades. Related references: PMID:1384049, PMID:1385158, PMID:2470098, PMID:7509083
• CD247 is specifically expressed in T-lymphocytes and natural killer cells, with high expression in peripheral blood mononuclear cells and lymphoid tissues. Related references: PMID:11722641
• As a transmembrane protein, CD3H localizes to the cell membrane, where it associates with the TCR and other CD3 subunits (CD3D, CD3E, CD3G).
• Plays an important role in intrathymic T-cell differentiation and in activity-dependent synapse formation of retinal ganglion cells (by similarity).
• The protein is a phosphoprotein and undergoes alternative splicing; it also participates in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic domain (aa 50-164), suggesting detection of intracellular epitopes; ensure appropriate permeabilization for IF/ICC and IHC-P protocols.
• For Western blot, note the predicted molecular weight of 19 kDa and select gel percentage and transfer conditions accordingly; use positive control lysates from human, mouse, or rat T-cell lines.
• Due to the polyclonal nature of the antibody, lot-to-lot consistency may vary; validate each new lot for your specific application and include appropriate negative controls.
• Optimize blocking and antibody dilutions to minimize non-specific binding, particularly when detecting low-abundance targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to provide a seamless supply of IVD raw materials. This anti-CD3H antibody supports T-cell and immuno-oncology research, and we offer complementary validated antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets. From concept to clinic, CamelBio accelerates your diagnostic development with reliable, high-quality materials and technical support.