Anti-CD3H (CD247) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P20963

Core Product Specifications and Parameters

Biological Background: CD3H (CD247) Function and Localization

• CD3H, also known as CD247 or T-cell receptor T3 zeta chain, is the zeta subunit of the TCR-CD3 complex, essential for adaptive immune response and T-cell signal transduction.
• The cytoplasmic domain contains immunoreceptor tyrosine-based activation motifs (ITAMs) that become phosphorylated by Src family kinases LCK and FYN upon TCR engagement, initiating downstream signaling. Related references: PMID:1384049, PMID:1385158, PMID:2470098
• Phosphorylated ITAMs serve as docking sites for the protein kinase ZAP70, leading to ZAP70 phosphorylation and conversion into a catalytically active enzyme, a critical step in T-cell activation. Related references: PMID:7509083
• CD247 plays an important role in intrathymic T-cell differentiation, influencing T-cell development in the thymus.
• It also participates in activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
• Subcellularly, CD247 localizes to the cell membrane.
• CD247 is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs). Related references: PMID:11722641
• Keywords: Adaptive immunity, alternative splicing, host-virus interaction, phosphoprotein, transmembrane, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 50–164) covers the intracellular ITAM-containing region; thus, the antibody targets the cytoplasmic domain of CD247.
• For WB, a band around 19 kDa is expected under reducing conditions; using Jurkat T-cell lysate as a positive control is advisable.
• In IHC-P, antigen retrieval and permeabilization may be necessary to expose the intracellular epitope.
• For ELISA, the antibody can be used for detection; optimal concentrations should be determined in the user's assay system.
• Cross-reactivity with mouse and rat CD247 makes it suitable for rodent models, but confirm sequence homology for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. For T-cell biology and immune synapse research, we offer validated monoclonal antibodies like this anti-CD247 clone, along with bulk ancillary reagents, antibody pairs, and custom rare-target development. Our integrated supply chain and technical consultation streamline procurement and accelerate assay development.