Anti-CD3G Rabbit Monoclonal Antibody for WB - P09693

Core Product Specifications and Parameters

Biological Background: CD3G Function and Localization

• CD3G is the gamma chain of the CD3 T-cell co-receptor, a member of the immunoglobulin superfamily, and a core component of the TCR-CD3 complex on T-lymphocytes.
• The TCR-CD3 complex is central to adaptive immunity; upon antigen recognition, CD3 chains (CD3D, CD3E, CD3G, and CD247) transduce activation signals via their cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). These ITAMs are phosphorylated by Src family kinases LCK and FYN, initiating downstream signaling cascades (PubMed:2470098). Related references: PMID:2470098
• CD3G is required for the dynamic regulation of TCR surface expression. Its cytoplasmic domain contains a di-leucine-based (diL) sorting motif that controls constitutive TCR cycling, ensuring proper receptor levels on the plasma membrane (PubMed:8187769). Related references: PMID:8187769
• The protein is a single-pass type I membrane protein localized to the cell membrane, with a signal peptide, an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail.
• Key post-translational modifications include phosphorylation of ITAM tyrosines upon T-cell activation, making it a phosphoprotein.
• CD3G is expressed on T-lymphocytes and plays a critical role in T-cell development and function; mutations are associated with immunodeficiency (IMD17).
• It contains disulfide bonds and is a glycoprotein, with a molecular weight of approximately 20 kDa (calculated 20,469 Da from UniProt; observed ~20 kDa by SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen was designed against a synthetic peptide corresponding to amino acids 83–182 of human CD3G (P09693), covering part of the extracellular domain and potentially the transmembrane region. This region is highly conserved, supporting the reported cross-reactivity with mouse.
• For Western blotting, expect a specific band at approximately 20 kDa under reducing conditions. Consider using lysates from T-cell lines (e.g., Jurkat) as positive controls.
• In IHC-P and IF/ICC, optimize antigen retrieval and permeabilization protocols, and validate membrane staining patterns in T-cell-rich tissues (e.g., lymph node, spleen).
• For ELISA, evaluate the antibody as a capture or detection reagent after confirming reactivity in your assay system.
• As with any antibody, perform appropriate controls and titrate the antibody to determine optimal working dilutions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop partner for IVD raw material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target material procurement. This CD3G Rabbit mAb supports T-cell biology and immune function research, offering reliable performance in core immunoassay platforms. From concept to clinic, CamelBio bridges the gap between diagnostic manufacturers, laboratories, and high-quality raw materials.