Anti-CD3G Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09693

Core Product Specifications and Parameters

Biological Background: CD3G Function and Localization

• CD3G is the gamma chain of the T-cell surface glycoprotein CD3, also known as T-cell receptor T3 gamma chain, and is part of the TCR-CD3 complex essential for adaptive immunity.
• The CD3 chains (CD3D, CD3E, CD3G, and CD247) transmit TCR-mediated signals across the cell membrane upon antigen recognition, containing immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains. Related references: PMID:2470098
• Upon TCR engagement, ITAMs are phosphorylated by Src family kinases LCK and FYN, leading to activation of downstream signaling pathways involved in T-cell activation.
• CD3G plays an additional role in the dynamic regulation of TCR expression at the cell surface via a di-leucine-based (diL) receptor-sorting motif present in its cytoplasmic tail. Related references: PMID:8187769
• The protein is a single-pass type I membrane protein localized at the cell membrane, with an extracellular immunoglobulin-like domain and a cytoplasmic tail containing the ITAM and diL motifs.
• CD3G undergoes post-translational modifications including phosphorylation, disulfide bond formation, and glycosylation, which are critical for its stability and function.
• It is involved in adaptive immune responses and is a key component of the T-cell receptor complex, serving as a receptor for antigen recognition.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions; CD3G runs at approximately 20-25 kDa due to glycosylation, so validate band size with appropriate positive controls (e.g., Jurkat cell lysate).
• In IHC-P and IF/ICC, test on human lymphoid tissues or T-cell lines, and optimize antigen retrieval and permeabilization conditions as needed.
• For ELISA, pair this antibody with a well-characterized detection reagent and titrate both components using recombinant CD3G protein or cell lysates.
• Since the immunogen covers the extracellular domain (aa 23-116), this antibody is likely suitable for detecting surface-expressed CD3G in live-cell applications; confirm staining in non-permeabilized cells.
• Cross-reactivity with other species is not documented; if using non-human samples, validate specificity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from early-stage discovery to clinical application. For T-cell biology and immunology studies, we offer this validated anti-CD3G monoclonal antibody alongside optimized antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities. Whether you need consistent lot-to-lot performance or custom supply arrangements, CamelBio provides reliable access to critical reagents that accelerate your assay development.