Anti-CD3E Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IF-P, IHC-P, FC, ELISA - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• CD3E encodes the CD3 epsilon chain, a core subunit of the T-cell receptor (TCR)-CD3 complex on the T-lymphocyte surface, essential for adaptive immune responses. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• The TCR-CD3 complex transduces antigen recognition signals via CD3 chains (CD3D, CD3E, CD3G, and CD247/CD3Z), each containing immunoreceptor tyrosine-based activation motifs (ITAMs) that are phosphorylated by Src kinases LCK and FYN upon TCR engagement. Related references: PMID:2470098 PMID:40592325
• Phosphorylated CD3E ITAMs recruit and activate ZAP70 kinase, initiating downstream signaling cascades critical for T-cell activation and development.
• CD3E also mediates internalization and down-regulation of surface TCR-CD3 complexes via cytosolic endocytosis sequences, regulating signal strength. Related references: PMID:10384095 PMID:26507128
• Beyond its coreceptor function, CD3E acts as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 interaction, disrupting ZAP70 binding and blocking ERK-NFkB signaling and calcium influx. Related references: PMID:12110186 PMID:38614099
• The protein localizes to the cell membrane, with a single-pass transmembrane helix and an extracellular immunoglobulin domain stabilized by a disulfide bond.
• CD3E undergoes phosphorylation and is classified under adaptive immunity, receptor, and membrane keywords, reflecting its central role in immune cell biology.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-126) of human CD3E, which includes the extracellular domain. This design is expected to recognize the mature cell surface form of CD3 epsilon in native and denaturing conditions.
• As a polyclonal antibody, it may detect multiple epitopes, providing robust signals in applications such as WB, IP, and IHC-P. Consider using appropriate blocking buffers (e.g., 5% non-fat dry milk) and validate in relevant T-cell lines or primary lymphocytes.
• For FC, use an isotype control to differentiate specific binding due to the polyclonal nature. Optimize antibody dilution for each application, starting with 1:500-1:2000 for WB and 1:50-1:200 for IHC/IF.
• Cross-reactivity with mouse and rat CD3E is indicated, but sequence alignment of the immunogen region is recommended to confirm epitope conservation across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with comprehensive IVD raw material solutions, from validated antibody pairs to bulk ancillary reagents. For T-cell biology and immunology projects, we offer a wide range of CD3E antibodies, including monoclonal and polyclonal options, optimized for diverse assay platforms. Beyond catalog products, our technical team assists in sourcing rare targets and custom raw materials, streamlining your path from assay concept to clinical application.