Anti-CD3E Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• The CD3E gene encodes the epsilon chain of the CD3 T-cell co-receptor, a key component of the TCR-CD3 complex on T lymphocytes that is essential for adaptive immune responses (PubMed:15294938, PubMed:15546002, PubMed:2470098).
  Related references: PMID:15294938, PMID:15546002, PMID:2470098
• Upon TCR engagement by antigen-presenting cells, CD3E transduces signals through its cytoplasmic ITAM motifs, which are phosphorylated by LCK and FYN kinases, leading to ZAP70 recruitment and downstream pathway activation (PubMed:2470098, PubMed:40592325).
  Related references: PMID:2470098, PMID:40592325
• CD3E is indispensable for proper T-cell development and maturation in the thymus (By similarity).
• The cytosolic region of CD3E contains endocytosis sequences that mediate internalization and cell surface down-regulation of TCR-CD3 complexes, regulating receptor availability (PubMed:10384095, PubMed:26507128).
  Related references: PMID:10384095, PMID:26507128
• CD3E also serves as a receptor for ITPRIPL1, which upon binding inhibits T-cell activation by promoting NCK1 association, thereby blocking ZAP70 interaction and dampening ERK-NFkB and calcium signaling (PubMed:12110186, PubMed:38614099).
  Related references: PMID:12110186, PMID:38614099
• CD3E is a transmembrane glycoprotein with an extracellular immunoglobulin-like domain and a cytoplasmic ITAM motif, classified as a single-pass type I membrane protein.
• It localizes predominantly to the cell membrane of T lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the central region (aa 111–207) of human CD3E; expect cross-reactivity with human, mouse, and rat samples based on sequence homology.
• For western blotting (WB), the predicted molecular weight is approximately 23 kDa; consider optimizing blocking and antibody dilution for your specific sample matrix.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization may be required; validate staining in T-cell-rich tissues or cell lines.
• For ELISA, pair this polyclonal antibody with a suitable capture antibody or standard; perform titration to determine optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing diagnostic developers and researchers with reliable access to antibodies, recombinant proteins, and ancillary reagents. For T-cell immunology targets such as CD3E, we offer validated polyclonal antibodies, matched pairs, and bulk production capabilities to support your assay development and scaling needs. Our integrated supply chain and technical support aim to streamline your journey from concept to clinic.