Anti-CD3E Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - P07766

Core Product Specifications and Parameters

Biological Background: CD3E Function and Localization

• CD3E is the epsilon chain of the T-cell surface glycoprotein CD3, which forms the TCR-CD3 complex on T lymphocytes and plays an essential role in adaptive immune responses. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• The CD3 chains (CD3D, CD3E, CD3G, CD247) contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains. Upon TCR engagement by antigen-MHC, these ITAMs are phosphorylated by Src kinases LCK and FYN, creating docking sites for ZAP70 and triggering downstream signaling cascades. Related references: PMID:2470098, PMID:40592325
• Beyond signal transduction, CD3E is essential for proper T-cell development in the thymus (By similarity).
• CD3E participates in the internalization and down-regulation of cell surface TCR-CD3 complexes through endocytosis motifs in its cytosolic tail. Related references: PMID:10384095, PMID:26507128
• CD3E also functions as a receptor for ITPRIPL1, a negative regulator that inhibits T-cell activation by promoting NCK1 binding, thereby preventing CD3E-ZAP70 interaction and blocking ERK-NFkB signaling and calcium influx. Related references: PMID:38614099, PMID:12110186
• Subcellular location: Cell membrane; single-pass type I membrane protein.
• Predicted molecular weight: ~23 kDa; consistent with the 23,147 Da calculated from sequence.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band at approximately 23 kDa may be detected under reducing conditions. Consider optimizing antibody concentration and incubation conditions for your sample type.
• For immunohistochemistry (IHC) and immunofluorescence (IF), heat-induced antigen retrieval in appropriate buffer may be beneficial. Validate staining in positive control tissues (e.g., human tonsil or lymph node).
• When including this antibody in multiplex IHC (mIHC) panels, verify compatibility with other primary antibodies and detection systems.
• Cross-reactivity with mouse and rat has been observed; hence, this antibody can be utilized in rodent model studies; however, confirm performance with your specific samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting diagnostic developers from concept to clinic. For CD3E-related immunology or oncology biomarker projects, we offer validated antibody pairs, recombinant proteins, and ancillary reagents in bulk quantities. Contact our team to discuss customized procurement and technical support for your diagnostic assay development.