Anti-CD3D Rabbit Monoclonal Antibody for WB, IF-P, FC, ELISA - P04234

Core Product Specifications and Parameters

Biological Background: CD3D Function and Localization

• CD3D encodes the delta chain of the T-cell surface glycoprotein CD3, a core component of the TCR-CD3 complex present on T lymphocytes. It is essential for adaptive immune response: upon TCR engagement by antigen-presenting cells, CD3 chains transmit signals across the cell membrane via their immunoreceptor tyrosine-based activation motifs (ITAMs), leading to activation of downstream signaling pathways.
  Related references:
  PMID:2470098
• CD3D plays a crucial role in thymocyte differentiation, ensuring correct intracellular assembly and surface expression of the TCR-CD3 complex. It also interacts with CD4 and CD8 co-receptors to facilitate T-cell activation and positive selection.
  Related references:
  PMID:12215456
• Subcellular localization: Cell membrane.
• Tissue specificity: predominantly expressed on T lymphocytes and also found in fetal NK cells.
• Alternative splicing generates multiple isoforms.
• Keywords: adaptive immunity, glycoprotein, phosphoprotein, transmembrane helix, receptor, signaling, SCID (severe combined immunodeficiency) associated.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 72–171 of human CD3D; this peptide-based design may favor recognition of denatured protein in Western blot (WB) applications. Consider using low molecular weight markers and optimal transfer conditions for the 19 kDa target.
• For immunofluorescence (IF-P) and flow cytometry (FC), ensure proper permeabilization and fixation protocols depending on whether the epitope is intracellular or surface-accessible. Validate the antibody in relevant T-cell lines (e.g., Jurkat) or primary human PBMCs.
• In ELISA, pair with a capture/detection antibody validated for the CD3D target to build a sensitive sandwich assay. Cross-reactivity with other species has not been tested; use human samples only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD3D monoclonal antibody supports T-cell immunology research and clinical diagnostics; we also supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and perform rare target raw-material sourcing tailored to your IVD assay development needs.