Anti-CD3d Polyclonal Antibody for WB, ELISA - P04235

Core Product Specifications and Parameters

Biological Background: CD3d Function and Localization

• CD3d is the delta chain of the T-cell receptor-CD3 complex, which plays an essential role in adaptive immune responses.
• Upon TCR engagement by antigen-presenting cells, the CD3 chains (including CD3d) transmit signals across the membrane via immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains.
• ITAM phosphorylation by Src-family kinases LCK and FYN initiates downstream signaling cascades that drive T-cell activation.
• CD3d is critical for thymocyte differentiation: it ensures correct TCR-CD3 complex assembly and surface expression, and its absence impairs thymocyte development (PubMed:10935641). Related references: PMID:10935641
• CD3d interacts with the coreceptors CD4 and CD8, establishing a functional link between TCR and these molecules that is required for T-cell activation and positive selection.
• Subcellular localization is the plasma membrane; it is a single-pass type I membrane protein.
• Classified as a glycoprotein, phosphoprotein, and receptor, with a disulfide bond and a transmembrane helix.

Experimental Guidance and Technical Tips

• For western blotting, consider using whole‑cell lysates from mouse T‑cell lines or primary lymphocytes under reducing conditions and validate with appropriate controls.
• The immunogen spans the extracellular region (aa 15–115); thus, the antibody may recognize both native and denatured CD3d, making it suitable for ELISA applications where native conformation could be relevant.
• Optimize antibody dilution and incubation conditions in your specific experimental system, as polyclonal antibodies may exhibit lot‑to‑lot variability.
• Not recommended for applications beyond those listed without further end‑user validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw‑material procurement by providing one‑stop access to validated antibodies, including this anti‑CD3d polyclonal antibody, alongside bulk ancillary reagents and rare‑target sourcing. Whether you need optimized monoclonal/polyclonal pairs, technical consultations, or scaled‑up production support, our supply chain accelerates your T‑cell biology and immunology‑based assay development from concept to clinic.