Anti-CD39/ENTPD1 Monoclonal Antibody for FC, ELISA - P49961

Core Product Specifications and Parameters

Biological Background: CD39/ENTPD1 Function and Localization

• CD39/ENTPD1 (ectonucleoside triphosphate diphosphohydrolase 1) is a transmembrane enzyme that sequentially hydrolyzes extracellular nucleoside triphosphates (NTPs) to diphosphates (NDPs) and then to monophosphates (NMPs), thus controlling the availability of signaling nucleotides. Related references: PMID:8529670 PMID:8626624 PMID:8955160
• By degrading pro-inflammatory ATP and platelet-activating ADP, CD39 inhibits platelet aggregation and promotes vascular homeostasis, underscoring its role in thromboregulation. Related references: PMID:8955160 PMID:8996251
• CD39 is highly expressed on activated lymphoid cells and endothelial tissues, with additional expression in placenta, lung, skeletal muscle, and kidney.
• The protein localizes to the plasma membrane and caveolae, consistent with its function in regulating extracellular nucleotide pools.
• It contains conserved ATP/GTP-binding domains, disulfide bonds, and multiple glycosylation sites, classifying it as a calcium- and magnesium-dependent hydrolase.
• Mutations in ENTPD1 are linked to hereditary spastic paraplegia type 64 (SPG64), highlighting its importance beyond the vasculature.
• Alternative splicing of ENTPD1 produces isoforms that may affect enzymatic activity or localization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 255–442 of the extracellular domain, making the antibody suitable for surface staining of live cells in flow cytometry without permeabilization.
• For ELISA, consider pairing with a different clone or using it as a capture antibody for soluble CD39, and validate with appropriate biological samples.
• Although validated for human reactivity, cross-reactivity with other species should be confirmed experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD39 rabbit mAb. Our portfolio covers antibodies for key nucleotidase targets, supporting vascular and thrombosis research. From bulk ancillary reagents to rare target sourcing, we simplify your supply chain from concept to clinic.