Anti-CD38 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P56528

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, with aliases including I-19, ADPRC 1, and NIM-R5 antigen.
• It synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion, and also produces nicotinate-adenine dinucleotide phosphate (NAADP+) from 2'-phospho-cADPR and nicotinic acid. Related references: PMID:11829748
• CD38 functions as a receptor binding CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and migration across endothelial barriers.
• In dendritic cells, CD38 regulates crucial mature-stage functions such as CCL21-driven migration, survival, and Th1-polarizing activity.
• Subcellularly, CD38 is a membrane protein with a signal-anchor and transmembrane helix; it is a glycoprotein with disulfide bonds and possesses both hydrolase and transferase enzymatic activities.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 45–304, a large extracellular region; this design may yield antibodies recognizing native CD38 in mouse samples.
• For Western blotting, a band at approximately 34 kDa is anticipated; validate using mouse tissue or cell lysates known to express CD38.
• In IF/ICC, membrane staining should be observed; appropriate permeabilization may enhance detection.
• Flow cytometry can be performed on mouse lymphocyte populations; titrate the antibody to optimize the signal.
• ELISA protocols can be developed using either direct antigen coating or sandwich formats; consider pairing with other CD38-specific reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CD38-related projects—whether studying calcium signaling, lymphocyte biology, or developing immunoassays—contact us to leverage our sourcing expertise and accelerate your path from concept to clinic.