Anti-CD38 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein that functions as ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase, synthesizing the second messenger cyclic ADP-ribose (cADPR) from NAD+. Related references: PMID:7961800 PMID:8253715
• CD38 also catalyzes the synthesis of nicotinate-adenine dinucleotide phosphate (NAADP+), a potent Ca2+ mobilizer, preferentially from 2'-phospho-cADPR and nicotinic acid. Related references: PMID:16690024
• Beyond enzymatic activity, CD38 acts as a receptor for CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and transendothelial migration. In lamina propria T lymphocytes, CD38/CD31 interactions activate LCK and LAT leading to cytokine release. Related references: PMID:9551996 PMID:11259373
• CD38 regulates dendritic cell functions including CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598
• Subcellularly, CD38 is a type II transmembrane protein located on the cell surface, with its catalytic domain facing the extracellular space.
• CD38 is highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, and in malignant lymphoma and neuroblastoma.
• Post-translational modifications include glycosylation, disulfide bonds, and alternative splicing that generate isoforms with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 181–300 within the extracellular domain of human CD38, likely recognizing both glycosylated and unglycosylated forms; consider using PNGase F to confirm glycosylation status in your system.
• For Western blot, denaturing and reducing conditions are typically suitable; a band near 34 kDa is expected, though glycosylation may shift the apparent molecular weight.
• In IHC-P and IF/ICC, include appropriate antigen retrieval and permeabilization steps; validate staining in tissues with known high expression (e.g., pancreas, liver, lymph node).
• Since cross-reactivity is reported for mouse and rat, this antibody may be used in translational or comparative studies; validate in the relevant sample system.
• For ELISA, the polyclonal nature provides robust signal; consider pairing with a monoclonal capture antibody in a sandwich assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with critical reagents like this CD38 polyclonal antibody for immunology and metabolism research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development from concept to clinic. Our supply network supports consistent lot-to-lot performance and scalable quantities to meet your project needs.