Anti-CD38 Monoclonal Antibody () for FC - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1) is a multifunctional transmembrane glycoprotein encoded by the CD38 gene, with at least one known isoform generated by alternative splicing.
• Core enzymatic function: synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion, and also produces nicotinate-adenine dinucleotide phosphate (NAADP+), a Ca2+ mobilizer. Related references: PMID:7961800, PMID:8253715, PMID:16690024
• Acts as both ADP-ribosyl cyclase and cADPR hydrolase, regulating NAD+ metabolism and calcium signaling. Related references: PMID:7961800, PMID:8253715
• Serves as a receptor on lymphocytes, binding to CD31 on endothelial cells to promote lymphocyte activation, proliferation, and transendothelial migration. Related references: PMID:9551996
• Involved in the regulation of dendritic cell functions including CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598
• In lamina propria T lymphocytes, CD38/CD31 cognate interactions activate LCK and LAT, leading to cytokine release. Related references: PMID:11259373
• Subcellularly localized to the cell surface and cell membrane, consistent with its roles in signaling and adhesion.
• Highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, and in malignant lymphoma and neuroblastoma.
• Contains disulfide bonds and glycosylation sites, and features a transmembrane helix anchoring it to the membrane.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 43-300) corresponds to a large segment of the extracellular domain, suggesting that the antibody recognizes native cell surface CD38, making it suitable for flow cytometry applications.
• CD38 is abundantly expressed on plasma cells and activated lymphocytes; include appropriate positive (e.g., human PBMC) and negative (e.g., CD38-knockout) controls when designing experiments.
• For multicolor flow cytometry panels, verify spectral compatibility of (Excitation ~450 nm) with other fluorophores to avoid compensation issues.
• CD38 is subject to glycosylation and disulfide bond formation; treat cells gently and avoid harsh fixation or permeabilization that may alter epitope conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD manufacturers, providing pre-validated antibody raw materials like this anti-CD38 monoclonal antibody to support immunology and oncology diagnostic development. From optimized monoclonal and polyclonal antibodies to bulk ancillary reagents and rare target procurement, CamelBio streamlines your supply chain, helping bring assays from concept to clinic efficiently.