Anti-CD36/SR-B3 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P16671

Core Product Specifications and Parameters

Biological Background: CD36/SR-B3 Function and Localization

• Multifunctional receptor: Acts as a receptor for diverse ligands such as thrombospondin, collagen, oxidized LDL, long-chain fatty acids, and amyloid-beta, mediating angiogenesis, inflammation, and metabolism.
• Fatty acid transport: Facilitates cellular uptake of long-chain fatty acids via a palmitoylation-regulated mechanism; palmitoylated CD36 captures fatty acids, then depalmitoylation triggers endocytic delivery. This process is crucial for muscle lipid utilization, adipose energy storage, and gut absorption. Related references: PMID:18353783 PMID:21395585 PMID:21610069 PMID:32958780 PMID:18753675
• Taste perception: Involved in oral fat perception and preferences; in taste cells, mediates long-chain fatty acid-induced calcium signaling and neuronal activation. Related references: PMID:22240721 PMID:25822988
• Angiogenesis regulation: Receptor for thrombospondins THBS1 and THBS2, mediating antiangiogenic effects; also promotes podocyte apoptosis in response to free fatty acids.
• Inflammatory signaling: As a coreceptor for TLR4:TLR6, recognizes oxidized LDL and amyloid-beta, triggering NF-κB-dependent inflammatory cytokine production and NLRP3 inflammasome activation. Related references: PMID:20037584
• Microbial sensing: Selective sensor of microbial diacylated lipopeptides via TLR2:TLR6 heterodimer, leading to TNF production and lipid-raft-dependent trafficking. Related references: PMID:16880211
• Subcellular localization: Present at the cell membrane, membrane rafts, Golgi apparatus, and apical cell membrane. Post-translational modifications include palmitoylation, glycosylation, disulfide bonds, and ubiquitination.
• Disease relevance: Alternative splicing produces isoforms; genetic variants are associated with platelet glycoprotein IV deficiency and metabolic syndrome.

Experimental Guidance and Technical Tips

• The immunogen spans the large extracellular domain (aa 30-439), which is highly conserved across species; the antibody is expected to recognize native CD36 in human, mouse, and rat samples.
• For Western blot, the predicted molecular weight is ~53 kDa; ensure adequate blocking due to the glycoprotein nature and consider using a high-quality blocking buffer.
• For immunohistochemistry (paraffin-embedded tissues), heat-induced antigen retrieval may be required; optimize the protocol for your specific tissue type.
• Validate the antibody in positive control cell lines or tissues known to express CD36, such as platelets, monocytes, adipocytes, or intestinal epithelium.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare targets like CD36/SR-B3, ensuring seamless integration into your assay development pipeline.