Anti-CD36 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q08857

Core Product Specifications and Parameters

Biological Background: CD36 Function and Localization

• CD36 (also known as Platelet glycoprotein 4, FAT, GPIV, Scarb3) is a multifunctional transmembrane glycoprotein receptor belonging to the class B scavenger receptor family.
• It binds a broad spectrum of ligands including thrombospondin, oxidized LDL, long-chain fatty acids, collagen, and microbial diacylated lipopeptides, triggering receptor clustering and downstream signaling (PubMed:18332148, PubMed:7685021).
  Related references:
  PMID:18332148 | PMID:7685021
• CD36 acts as a major fatty acid transporter, facilitating long-chain fatty acid uptake into cells via a dynamic palmitoylation–depalmitoylation cycle involving LYN kinase and ZDHHC5 (PubMed:18332148, PubMed:30605677, PubMed:32958780).
  Related references:
  PMID:18332148 | PMID:30605677 | PMID:32958780
• In innate immunity, CD36 serves as a coreceptor for TLR4:TLR6 and TLR2:TLR6 heterodimers, driving NF-κB-dependent inflammatory cytokine production and NLRP3 inflammasome activation in response to oxLDL, amyloid-beta, or bacterial lipopeptides (PubMed:20037584, PubMed:23812099).
  Related references:
  PMID:20037584 | PMID:23812099
• As a receptor for thrombospondins (THBS1 and THBS2), CD36 mediates antiangiogenic effects and promotes podocyte apoptosis under elevated free fatty acid conditions (PubMed:15748999, PubMed:25835637).
  Related references:
  PMID:15748999 | PMID:25835637
• In the small intestine, CD36 is strongly expressed on the luminal surface of enterocytes in a proximal-to-distal gradient and is critical for proximal absorption of dietary fatty acids and cholesterol for chylomicron formation (PubMed:17507371, PubMed:18753675, PubMed:21610069).
  Related references:
  PMID:17507371 | PMID:18753675 | PMID:21610069
• Subcellularly, CD36 localizes to the cell membrane, apical membrane, membrane rafts, and Golgi apparatus, consistent with its roles in ligand binding, trafficking, and signaling.
• CD36 is expressed in taste bud cells of the circumvallate papillae, where it detects long-chain fatty acids and triggers intracellular calcium increases and gustatory neuron activation (PubMed:16276419, PubMed:18162488).
  Related references:
  PMID:16276419 | PMID:18162488
• As a pattern recognition receptor, CD36 directly mediates adhesion and internalization of Plasmodium falciparum-parasitized erythrocytes and uptake of bacteria such as E.coli and S.aureus (PubMed:19864601, PubMed:23395392, PubMed:16020694).
  Related references:
  PMID:19864601 | PMID:23395392 | PMID:16020694

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–439 of mouse CD36, covering a large portion of the extracellular domain; this region shares high homology with rat CD36, consistent with the observed cross-reactivity.
• In Western blot, a principal band around 53 kDa is predicted; however, glycosylation and other modifications may result in higher apparent molecular weights. Use reducing conditions and appropriate protein markers.
• For IF/ICC and IHC-P, CD36 immunoreactivity is expected at the plasma membrane and possibly Golgi; include relevant controls such as macrophage-rich tissues or known positive cell lines.
• Given its polyclonal nature, this antibody may be used to capture CD36 in ELISA; for sandwich applications, consider pairing with a monoclonal CD36 antibody.
• Validate specificity in your sample system, especially when working with complex tissues, by including knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized procurement for niche targets. The CD36 antibody fits into our portfolio for lipid metabolism, inflammation, and infectious disease research, and we can supply complementary reagents such as recombinant CD36 proteins, ELISA kits, and blocking buffers. From early-stage feasibility to clinical applications, CamelBio provides reliable, single-channel access to high-quality materials and technical support to accelerate your diagnostic development.