Anti-CD35/CR1 Polyclonal Antibody for WB, IF/ICC, ELISA - P17927

Core Product Specifications and Parameters

Biological Background: CD35/CR1 Function and Localization

• CD35/CR1, also known as complement receptor type 1 or C3b/C4b receptor, is a large membrane glycoprotein of approximately 224 kDa that belongs to the regulators of complement activation (RCA) family (SWISS: P17927).
• It functions as an immune adherence receptor, mediating the capture and clearance of complement-opsonized pathogens and immune complexes by erythrocytes and phagocytes (PubMed:2963069). Related references: PMID:2963069
• CD35 inhibits spontaneous complement activation by accelerating the decay of C3/C5 convertases of both alternative and classical pathways, and serves as a cofactor for factor I-mediated cleavage of C3b and C4b (PubMed:2972794, PubMed:8175757). Related references: PMID:2972794 PMID:8175757
• Upon ligand binding, CD35 contributes to the generation of regulatory T cells from activated helper T cells, highlighting its role in immune regulation (PubMed:25742728). Related references: PMID:25742728
• It also acts as a receptor for Epstein-Barr virus, facilitating viral entry into host cells.
• CD35 is primarily localized on the plasma membrane and is expressed on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes, and granulocytes.
• The protein is characterized by multiple short consensus repeat (SCR) domains (also known as sushi domains), extensive glycosylation, and disulfide bonds, and it carries the Knops blood group antigen system.
• Additional keywords: innate immunity, host-virus interaction, signal peptide, transmembrane helix.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human granulocytes or CD35-transfected cells; the expected band is around 224 kDa, and the polyclonal antibody recognizes an epitope within amino acids 420–550.
• For immunofluorescence/ICC, validate fixation and permeabilization conditions, as CD35 is a membrane protein; staining should localize to the cell surface.
• For ELISA, the antibody may be used for antigen capture or detection; optimal coating conditions and antibody concentration should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and technical support from concept to clinic. This anti-CD35/CR1 polyclonal antibody supports research in complement biology, immunology, and infectious disease, and can be integrated into assay development workflows. For specialized requirements such as matched antibody pairs, rare target sourcing, or custom manufacturing, CamelBio provides tailored end-to-end solutions to streamline your diagnostic development.