Anti-CD352/SLAMF6 Monoclonal Antibody for WB, IHC-P, FC, ELISA - Q96DU3

Core Product Specifications and Parameters

Biological Background: CD352/SLAMF6 Function and Localization

• SLAMF6 (also known as NTB-A, Ly108, CD352) is a member of the signaling lymphocytic activation molecule (SLAM) family, functioning as a self-ligand receptor that modulates both innate and adaptive immune responses through homo- or heterotypic cell-cell interactions.
• SLAMF6 triggers cytolytic activity specifically in natural killer (NK) cells that express high surface densities of natural cytotoxicity receptors. Related references: PMID:11489943 PMID:16920955
• Positive signaling in NK cells involves phosphorylation of VAV1 and is dependent on the adaptor protein SH2D1B/EAT-2. Related references: PMID:16920955
• In T lymphocytes, SLAMF6 promotes differentiation toward the Th17 phenotype, leading to increased IL-17 secretion via recruitment of RORC to the IL-17 promoter; this co‑stimulatory activity requires SH2D1A/SAP. Related references: PMID:16920955 PMID:22184727 PMID:22989874
• Together with SLAMF1 and CD84/SLAMF5, SLAMF6 may act as a negative regulator of the humoral immune response, and in the absence of SH2D1A/SAP it can transmit inhibitory signals to CD4+ T‑cells and NKT cells.
• SLAMF6 negatively regulates germinal center formation by inhibiting T‑cell:B‑cell adhesion; this function is associated with increased recruitment of the phosphatase PTPN6/SHP-1.
• The protein is a single‑pass type I membrane glycoprotein located at the cell membrane. It is expressed by all resting and activated NK cells, T‑cells, and B‑lymphocytes, with elevated surface expression reported on T‑cells from systemic lupus erythematosus (SLE) patients. Related references: PMID:11489943 PMID:22184727
• SLAMF6 undergoes alternative splicing, giving rise to multiple isoforms; it is modified by N‑linked glycosylation, disulfide bond formation, and phosphorylation on key tyrosine residues within its immunoreceptor tyrosine‑based switch motifs (ITSMs).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24–215) of human SLAMF6; the rabbit monoclonal antibody should recognize native surface‑expressed protein in flow cytometry and may be suitable for immunofluorescence and functional blocking studies.
• For Western blot, expect specific bands around 24 kDa (predicted for some isoforms) and 37 kDa (alternatively spliced/glycosylated forms); validate the observed molecular weights in your lysate.
• For immunohistochemistry (paraffin‑embedded tissue, IHC‑P), perform antigen retrieval with citrate buffer (pH 6.0) and optimize the primary antibody dilution using a known positive control tissue (e.g., tonsil or lymph node).
• For flow cytometry, use 0.5–1 µg of antibody per million cells in 100 µL staining volume; ensure viability as SLAMF6 is a surface marker.
• When setting up an ELISA detection system, pair with a matched capture/detection antibody or use this recombinant monoclonal as a detection reagent in a sandwich format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high‑quality IVD raw materials and technical services across the entire development pipeline. For immunology‑focused projects such as NK or T‑cell studies, we can supply validated antibody pairs, bulk quantities of this anti‑SLAMF6 monoclonal, as well as matched ancillary reagents. Our expertise also covers rare‑target sourcing and custom technical support to accelerate your assay development from concept to clinic.