Anti-CD34 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen, functioning as a possible adhesion molecule. It mediates attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells, facilitating early hematopoiesis.
• Acts as a scaffold for lineage-specific glycans, allowing stem cells to bind lectins on stromal cells or other marrow components. It also presents carbohydrate ligands to selectins.
• Localized to the membrane, consistent with its role in cell adhesion and signaling.
• Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of various tissues, highlighting its specificity.
• The protein is a glycoprotein with a signal peptide and a single transmembrane helix, and undergoes alternative splicing and phosphorylation.
• Keywords include Cell adhesion, Glycoprotein, Membrane, and Phosphoprotein, underscoring its structural and functional features.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 32-290 of human CD34, covering a large portion of the extracellular domain. This polyclonal antibody is expected to recognize multiple epitopes within this region, making it robust for detecting denatured or native CD34.
• For Western blotting (WB), a band around 41 kDa is anticipated. Reducing conditions and appropriate loading controls should be used, and membrane blocking optimized to minimize background.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary to unmask epitopes. Including tissue sections known to express CD34 (e.g., bone marrow or endothelial cells) is recommended for validation.
• For immunofluorescence (IF/ICC), permeabilization is required to access intracellular epitopes, though the extracellular domain may be detected without permeabilization in non-fixed live cells.
• In ELISA, the immunizing peptide or recombinant protein can serve as a positive control to confirm antibody reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers streamlined access to high-quality IVD raw materials, including this CD34 rabbit polyclonal antibody for stem cell and endothelial research. In addition to well-characterized antibodies, we supply matched pairs, bulk ancillary reagents, and can source custom raw materials for rare or emerging targets. Our integrated supply chain supports every stage from concept to clinic, ensuring consistency and compliance.