Anti-CD34 Rabbit Monoclonal Antibody for WB, IHC-P, mIHC, ELISA - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 (also known as GIG3, MORT1) is a hematopoietic progenitor cell antigen encoded by the CD34 gene.
• It functions as a possible adhesion molecule with a role in early hematopoiesis, mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• Acts as a scaffold for lineage-specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components; presents carbohydrate ligands to selectins.
• Subcellularly, CD34 is a membrane protein with a single transmembrane domain.
• It is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
• Classified under keywords: alternative splicing, cell adhesion, glycoprotein, membrane, phosphoprotein, and signal.
• The full-length protein has a calculated molecular weight of approximately 40.7 kDa; the product data lists 41 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular portion of CD34; thus the antibody likely detects total CD34 regardless of glycosylation state.
• For Western blotting, a band at approximately 41 kDa is expected; however, due to heavy glycosylation, mature CD34 often migrates at 90–120 kDa; validate migration in your sample.
• In immunohistochemistry (IHC-P), consider using heat-induced antigen retrieval for optimal staining; the antibody is suitable for paraffin-embedded sections.
• For multiplex IHC (mIHC), ensure compatibility of this rabbit monoclonal with other primary antibodies from different host species.
• Cross-reactivity with mouse and rat has been reported; validate in the relevant tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined, one-stop supply chain for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-CD34 clone, and bulk ancillary reagents. Our portfolio and services cover every stage from concept to clinic, supporting diagnostic manufacturers and research laboratories in accelerating assay development for hematopoietic and endothelial research. We also offer rare-target raw-material sourcing and tailored technical consulting to meet your project needs.